Which tubes served as negative controls in the experiment?

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Multiple Choice

Which tubes served as negative controls in the experiment?

Explanation:
Negative controls establish a baseline so we know that any signal comes from the tested variable, not from contamination or random noise. In this setup, the two tubes were prepared without the essential reactants needed for the reaction, so no product could form and the baseline remains unchanged. These tubes confirm that any change seen in the other tubes is due to the intended enzymatic activity, not some other factor.

Negative controls establish a baseline so we know that any signal comes from the tested variable, not from contamination or random noise. In this setup, the two tubes were prepared without the essential reactants needed for the reaction, so no product could form and the baseline remains unchanged. These tubes confirm that any change seen in the other tubes is due to the intended enzymatic activity, not some other factor.

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